RNA Complete BCT is a direct draw blood collection tube that maintains the draw time concentration of cell-free RNA and extracellular vesicles, such as exosomes. The preservative stabilizes cfRNA and exosome counts for up to 7 days when stored at room temperature, maximizing plasma yield and minimizing hemolysis during storage. By limiting the degradation of white and red blood cells, the specialized chemistry provides sample integrity during storage, shipping, and handling of blood samples.
Once isolated from stored plasma, the cfRNA and isolated extracellular vesicles are suitable for many downstream applications including qualitative and quantitative real-time PCR, Droplet Digital PCR, next-generation sequencing, and Nanoparticle Tracking Analysis. RNA Complete BCT is compatible with commercially available plasma nucleic acid isolation kits:
Qiagen QIAamp Circulating Nucleic Acid Kit, Norgen Plasma/Serum Circulating and Exosomal RNA Purification Kit, and Applied Biosystems MagMAX Cell-Free Total Nucleic Acid Isolation Kit. Designed for next-generation sequencing workflows, RNA Complete BCT maintains draw-time cfRNA transcript levels in whole blood samples for use in transcriptome studies. It is compatible with standard low input RNA library prep kits: New England BioLabs NEBNext Ultra II Directional RNA Library Prep Kit and Illumina TruSeq RNA Library Prep Kit.