The respiraScreen 1 real time RT-PCR Kit is a screening assay for the simultaneous detection of three groups of different respiratory viruses. The design allows the differentiation of Influenza Virus (Flu A, Flu B) and Respiratory Syncytial Virus (RSV A, RSV B) from the pandemic coronavirus SARS-CoV-2, extracted from biological specimens. The kit contains specific primers and dual-labeled probes for the amplification of RNA (cDNA) of Influenza A (M gene), Influenza B (NEP gene), RSV A (G gene), RSV B (G gene) and SARS-CoV-2 (E gene) extracted from biological specimen. Furthermore, the kit contains a Control RNA (Internal Process Control, IPC), which is added during RNA extraction and detected in the same reaction by a HEX-labelled probe. The Control RNA allows the detection of RT-PCR inhibition and acts as control that the nucleic acid was isolated from the biological specimen.