Características:
• Specific detection of IgG antibodies against SARS-CoV-2 using the S1 domain of the spike protein including the immunologically relevant receptor binding domain (RBD)
• RBD represents important target antigen for virus neutralizing antibodies
• Worldwide programs for vaccine development use the spike protein as antigen
• Available as ELISA for semi-quantitative and quantitative determination (same cut-off calibrator); quantitative results can NOW be given in standardized units!
• Excellent performance and good correlation with different test systems for the detection of neutralizing antibodies confirmed in external studies
• Validated for serum, plasma and dried capillary blood as sample material, complete automation possible
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Especificaciones:
• Antigen: S1 domain of the spike protein of SARS-CoV-2, recombinantly produced in human cells
• Calibration: Quantitative, in relative units per milliliter (RU/ml); simple calculation of results in binding antibody units per milliliter (BAU/ml)
Calibrator 1: 120 RU/ml; Calibrator 2: 80 RU/ml; Calibrator 3: 40 RU/ml; Calibrator 4: 20 RU/ml; Calibrator 5: 10 RU/ml; Calibrator 6: 1 RU/m
Recommended upper threshold of the reference range for non-infected individuals (cut-off): 10 RU/ml
• Sample dilution: Serum or plasma, 1 : 101 in sample buffer, or 4.76 mm membrane piece containing dried capillary blood (punched out from Blood Collection Card) in 250 μl sample buffer
• Reagents: Ready for use, with the exception of the wash buffer (10x); color-coded solutions, in most cases exchangeable with those in other EUROIMMUN ELISA kits
• Test procedure: 60 min (37 °C) / 30 min (37 °C) / 30 min (RT)(sample / conjugate / substrate incubations), fully automatable
• Measurement: 450 nm, reference wavelength between 620 nm and 650 nm
• Test kit format: 96 break-off wells; kit includes all necessary reagents
• Stability: 12 months
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